%0 Journal Article %J Cell %D 2021 %T Population-scale tissue transcriptomics maps long non-coding RNAs to complex disease. %A de Goede, Olivia M %A Nachun, Daniel C %A Ferraro, Nicole M %A Gloudemans, Michael J %A Rao, Abhiram S %A Smail, Craig %A Eulalio, Tiffany Y %A Aguet, Francois %A Ng, Bernard %A Xu, Jishu %A Barbeira, Alvaro N %A Castel, Stephane E %A Kim-Hellmuth, Sarah %A Park, YoSon %A Scott, Alexandra J %A Strober, Benjamin J %A Brown, Christopher D %A Wen, Xiaoquan %A Hall, Ira M %A Battle, Alexis %A Lappalainen, Tuuli %A Im, Hae Kyung %A Ardlie, Kristin G %A Mostafavi, Sara %A Quertermous, Thomas %A Kirkegaard, Karla %A Montgomery, Stephen B %K Coronary Artery Disease %K Diabetes Mellitus, Type 1 %K Diabetes Mellitus, Type 2 %K Disease %K Gene Expression Profiling %K Genetic Variation %K Humans %K Inflammatory Bowel Diseases %K Multifactorial Inheritance %K Organ Specificity %K Population %K Quantitative Trait Loci %K RNA, Long Noncoding %K Transcriptome %X

Long non-coding RNA (lncRNA) genes have well-established and important impacts on molecular and cellular functions. However, among the thousands of lncRNA genes, it is still a major challenge to identify the subset with disease or trait relevance. To systematically characterize these lncRNA genes, we used Genotype Tissue Expression (GTEx) project v8 genetic and multi-tissue transcriptomic data to profile the expression, genetic regulation, cellular contexts, and trait associations of 14,100 lncRNA genes across 49 tissues for 101 distinct complex genetic traits. Using these approaches, we identified 1,432 lncRNA gene-trait associations, 800 of which were not explained by stronger effects of neighboring protein-coding genes. This included associations between lncRNA quantitative trait loci and inflammatory bowel disease, type 1 and type 2 diabetes, and coronary artery disease, as well as rare variant associations to body mass index.

%B Cell %V 184 %P 2633-2648.e19 %8 2021 05 13 %G eng %N 10 %1 https://www.ncbi.nlm.nih.gov/pubmed/33864768?dopt=Abstract %R 10.1016/j.cell.2021.03.050 %0 Journal Article %J Genome Biol %D 2020 %T Impact of admixture and ancestry on eQTL analysis and GWAS colocalization in GTEx. %A Gay, Nicole R %A Gloudemans, Michael %A Antonio, Margaret L %A Abell, Nathan S %A Balliu, Brunilda %A Park, YoSon %A Martin, Alicia R %A Musharoff, Shaila %A Rao, Abhiram S %A Aguet, Francois %A Barbeira, Alvaro N %A Bonazzola, Rodrigo %A Hormozdiari, Farhad %A Ardlie, Kristin G %A Brown, Christopher D %A Im, Hae Kyung %A Lappalainen, Tuuli %A Wen, Xiaoquan %A Montgomery, Stephen B %X

BACKGROUND: Population structure among study subjects may confound genetic association studies, and lack of proper correction can lead to spurious findings. The Genotype-Tissue Expression (GTEx) project largely contains individuals of European ancestry, but the v8 release also includes up to 15% of individuals of non-European ancestry. Assessing ancestry-based adjustments in GTEx improves portability of this research across populations and further characterizes the impact of population structure on GWAS colocalization.

RESULTS: Here, we identify a subset of 117 individuals in GTEx (v8) with a high degree of population admixture and estimate genome-wide local ancestry. We perform genome-wide cis-eQTL mapping using admixed samples in seven tissues, adjusted by either global or local ancestry. Consistent with previous work, we observe improved power with local ancestry adjustment. At loci where the two adjustments produce different lead variants, we observe 31 loci (0.02%) where a significant colocalization is called only with one eQTL ancestry adjustment method. Notably, both adjustments produce similar numbers of significant colocalizations within each of two different colocalization methods, COLOC and FINEMAP. Finally, we identify a small subset of eQTL-associated variants highly correlated with local ancestry, providing a resource to enhance functional follow-up.

CONCLUSIONS: We provide a local ancestry map for admixed individuals in the GTEx v8 release and describe the impact of ancestry and admixture on gene expression, eQTLs, and GWAS colocalization. While the majority of the results are concordant between local and global ancestry-based adjustments, we identify distinct advantages and disadvantages to each approach.

%B Genome Biol %V 21 %P 233 %8 2020 09 11 %G eng %N 1 %1 https://www.ncbi.nlm.nih.gov/pubmed/32912333?dopt=Abstract %R 10.1186/s13059-020-02113-0 %0 Journal Article %J Science %D 2020 %T Transcriptomic signatures across human tissues identify functional rare genetic variation. %A Ferraro, Nicole M %A Strober, Benjamin J %A Einson, Jonah %A Abell, Nathan S %A Aguet, Francois %A Barbeira, Alvaro N %A Brandt, Margot %A Bucan, Maja %A Castel, Stephane E %A Davis, Joe R %A Greenwald, Emily %A Hess, Gaelen T %A Hilliard, Austin T %A Kember, Rachel L %A Kotis, Bence %A Park, YoSon %A Peloso, Gina %A Ramdas, Shweta %A Scott, Alexandra J %A Smail, Craig %A Tsang, Emily K %A Zekavat, Seyedeh M %A Ziosi, Marcello %A Ardlie, Kristin G %A Assimes, Themistocles L %A Bassik, Michael C %A Brown, Christopher D %A Correa, Adolfo %A Hall, Ira %A Im, Hae Kyung %A Li, Xin %A Natarajan, Pradeep %A Lappalainen, Tuuli %A Mohammadi, Pejman %A Montgomery, Stephen B %A Battle, Alexis %K Genetic Variation %K Genome, Human %K Humans %K Multifactorial Inheritance %K Organ Specificity %K Transcriptome %X

Rare genetic variants are abundant across the human genome, and identifying their function and phenotypic impact is a major challenge. Measuring aberrant gene expression has aided in identifying functional, large-effect rare variants (RVs). Here, we expanded detection of genetically driven transcriptome abnormalities by analyzing gene expression, allele-specific expression, and alternative splicing from multitissue RNA-sequencing data, and demonstrate that each signal informs unique classes of RVs. We developed Watershed, a probabilistic model that integrates multiple genomic and transcriptomic signals to predict variant function, validated these predictions in additional cohorts and through experimental assays, and used them to assess RVs in the UK Biobank, the Million Veterans Program, and the Jackson Heart Study. Our results link thousands of RVs to diverse molecular effects and provide evidence to associate RVs affecting the transcriptome with human traits.

%B Science %V 369 %8 2020 09 11 %G eng %N 6509 %1 https://www.ncbi.nlm.nih.gov/pubmed/32913073?dopt=Abstract %R 10.1126/science.aaz5900 %0 Journal Article %J Nature %D 2017 %T Genetic effects on gene expression across human tissues. %A Battle, Alexis %A Brown, Christopher D %A Engelhardt, Barbara E %A Montgomery, Stephen B %K Alleles %K Chromosomes, Human %K Disease %K Female %K Gene Expression Profiling %K Gene Expression Regulation %K Genetic Variation %K Genome, Human %K Genotype %K Humans %K Male %K Organ Specificity %K Quantitative Trait Loci %X

Characterization of the molecular function of the human genome and its variation across individuals is essential for identifying the cellular mechanisms that underlie human genetic traits and diseases. The Genotype-Tissue Expression (GTEx) project aims to characterize variation in gene expression levels across individuals and diverse tissues of the human body, many of which are not easily accessible. Here we describe genetic effects on gene expression levels across 44 human tissues. We find that local genetic variation affects gene expression levels for the majority of genes, and we further identify inter-chromosomal genetic effects for 93 genes and 112 loci. On the basis of the identified genetic effects, we characterize patterns of tissue specificity, compare local and distal effects, and evaluate the functional properties of the genetic effects. We also demonstrate that multi-tissue, multi-individual data can be used to identify genes and pathways affected by human disease-associated variation, enabling a mechanistic interpretation of gene regulation and the genetic basis of disease.

%B Nature %V 550 %P 204-213 %8 2017 Oct 11 %G eng %N 7675 %1 https://www.ncbi.nlm.nih.gov/pubmed/29022597?dopt=Abstract %R 10.1038/nature24277 %0 Journal Article %J Nature %D 2017 %T The impact of rare variation on gene expression across tissues. %A Li, Xin %A Kim, Yungil %A Tsang, Emily K %A Davis, Joe R %A Damani, Farhan N %A Chiang, Colby %A Hess, Gaelen T %A Zappala, Zachary %A Strober, Benjamin J %A Scott, Alexandra J %A Li, Amy %A Ganna, Andrea %A Bassik, Michael C %A Merker, Jason D %A Hall, Ira M %A Battle, Alexis %A Montgomery, Stephen B %K Bayes Theorem %K Female %K Gene Expression Profiling %K Genetic Variation %K Genome, Human %K Genomics %K Genotype %K Humans %K Male %K Models, Genetic %K Organ Specificity %K Sequence Analysis, RNA %X

Rare genetic variants are abundant in humans and are expected to contribute to individual disease risk. While genetic association studies have successfully identified common genetic variants associated with susceptibility, these studies are not practical for identifying rare variants. Efforts to distinguish pathogenic variants from benign rare variants have leveraged the genetic code to identify deleterious protein-coding alleles, but no analogous code exists for non-coding variants. Therefore, ascertaining which rare variants have phenotypic effects remains a major challenge. Rare non-coding variants have been associated with extreme gene expression in studies using single tissues, but their effects across tissues are unknown. Here we identify gene expression outliers, or individuals showing extreme expression levels for a particular gene, across 44 human tissues by using combined analyses of whole genomes and multi-tissue RNA-sequencing data from the Genotype-Tissue Expression (GTEx) project v6p release. We find that 58% of underexpression and 28% of overexpression outliers have nearby conserved rare variants compared to 8% of non-outliers. Additionally, we developed RIVER (RNA-informed variant effect on regulation), a Bayesian statistical model that incorporates expression data to predict a regulatory effect for rare variants with higher accuracy than models using genomic annotations alone. Overall, we demonstrate that rare variants contribute to large gene expression changes across tissues and provide an integrative method for interpretation of rare variants in individual genomes.

%B Nature %V 550 %P 239-243 %8 2017 10 11 %G eng %N 7675 %1 https://www.ncbi.nlm.nih.gov/pubmed/29022581?dopt=Abstract %R 10.1038/nature24267