@article {52,
	title = {Differential NOVA2-Mediated Splicing in Excitatory and Inhibitory Neurons Regulates Cortical Development and Cerebellar Function.},
	journal = {Neuron},
	volume = {101},
	year = {2019},
	month = {2019 Feb 20},
	pages = {707-720.e5},
	abstract = {<p>RNA-binding proteins (RBPs) regulate genetic diversity, but the degree to which they do so in individual cell types in~vivo is unknown. We developed NOVA2 cTag-crosslinking and immunoprecipitation (CLIP) to generate functional RBP-RNA maps from different neuronal populations in the mouse brain. Combining cell type datasets from Nova2-cTag and Nova2 conditional knockout mice revealed differential NOVA2 regulatory actions on alternative splicing (AS) on the same transcripts expressed in different neurons. This includes functional differences in transcripts expressed in cortical and cerebellar excitatory versus inhibitory neurons, where we find NOVA2 is required for, respectively, development of laminar structure, motor coordination, and synapse formation. We also find that NOVA2-regulated AS is coupled to NOVA2 regulation of intron retention in hundreds of transcripts, which can sequester the trans-acting splicing factor PTBP2. In summary, cTag-CLIP complements single-cell RNA sequencing (RNA-seq) studies by providing a means for understanding RNA regulation of functional cell diversity.</p>},
	keywords = {Alternative Splicing, Animals, Antigens, Neoplasm, Cells, Cultured, Cerebellum, Cerebral Cortex, Excitatory Postsynaptic Potentials, Female, Inhibitory Postsynaptic Potentials, Male, Mice, Mice, Inbred C57BL, Nerve Tissue Proteins, Neuro-Oncological Ventral Antigen, Neurogenesis, Neurons, Polypyrimidine Tract-Binding Protein, RNA-Binding Proteins},
	issn = {1097-4199},
	doi = {10.1016/j.neuron.2018.12.019},
	author = {Saito, Yuhki and Yuan, Yuan and Zucker-Scharff, Ilana and Fak, John J and Jereb, Sa{\v s}a and Tajima, Yoko and Licatalosi, Donny D and Darnell, Robert B}
}
